Urology Research & Practice
UROONCOLOGY - Original Article

Investigation of the Roles of MTHFR (C677T and A1298C) and MMP-2 (–1306C>T ) Variations in Bladder Cancer Development

1.

Department of Biophysics, Haliç University Faculty of Medicine, İstanbul, Turkey

2.

Department of Biophysics, Trakya University Faculty of Medicine, Edirne, Turkey

Urol Res Pract 2023; 49: 33-39
DOI: 10.5152/tud.2023.22185
Read: 377 Downloads: 150 Published: 10 March 2023

Objective: Bladder cancer is a complex malignancy and has been associated with high morbidity. Since susceptibility to bladder cancer development differs between individuals, determining the roles of MTHFR and MMP-2 gene variations associated with this cancer is important for analyzing differences in individual susceptibility. In this study, we aimed to investigate the role of MTHFR and MMP-2 gene variations in the development of bladder cancer in the Thrace region of Turkey.

Materials and methods: One hundred seventy-nine blood samples were collected, including 98 patients with bladder cancer and 81 healthy controls. DNA extraction was carried out with blood samples. Polymerase chain reaction-restriction fragment length polymorphism was applied to detect MTHFR C677T (rs 1801133), MTHFR A1298C (rs 1801131), and MMP-2 (–1306C>T) (rs 243865) gene variants.

Results: For the MTHFR A1298C gene variation, CC genotype was the genetic risk factor (P=.0001), while AC genotype was the protective factor (P< .0001) in the development of bladder cancer. For the MMP-2 (–1306C>T) gene variation, TT genotype (P < .0001) and T allele (P=.0006) were genetic risk factors, while AC genotype (P=.0009) was the protective factor in the development of bladder cancer. For C677T/A1298C gene variations, CC–CC combined genotype was the genetic risk factor (P=.009), while CT–AC and CC–AC combined genotypes were potential protective biomarkers (P=.013 and P < .001, respectively).

Conclusion: In our study, TT genotype and T allele were determined as genetic risk factors for MMP-2 (–1306C>T) gene variation. For C677T/A1298C gene variations, CC– CC combined genotype was detected as the genetic risk factor in the development of bladder cancer.

Cite this article as: Alkanli N, Ay A. Investigation of the roles of MTHFR (C677T and A1298C) and MMP-2 (–1306C>T) variations in bladder cancer development. Urol Res Pract., 2023;49(1):33-39.

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