Urology Research & Practice
Editorial

Relationship between testicular sperm extraction results and AZF gene mutations

1.

Bucak Devlet Hastanesi, Üroloji Kliniği, Burdur

2.

Ege Üniversitesi Tıp Fakültesi, Tıbbi Genetik Anabilim Dalı, İzmir

3.

Ege Üniversitesi Tıp Fakültesi, Üroloji Anabilim Dalı, İzmir

4.

Ege Üniversitesi Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, İzmir

Urol Res Pract 2011; 37: 229-234
Read: 1329 Downloads: 1025 Published: 25 July 2019

Abstract

Objective: In this study, the effects of testicular sperm extraction (TESE) outcome on Y microdeletion, caryotype analysis, and pathology results in azoospermic infertile male patients were investigated.

Materials and methods: A total of 165 azoospermic infertile male patients who underwent TESE between January 2008-December 2009 in Ege University Faculty of Medicine, Urology Department have been evaluated for medical history, physical examination, spermiogram, caryotype analysis, Y chromosome microdeletion, FSH level, and TESE outcome regarding sperm availability.

Results: Mean age of patients was 34±5.42 years, mean time of infertility was 73±57.7 months, and infertility type was primary in 98.8% of cases. Mean testicular volume was 12.3 cc, and varicocele was detected in 32 patients. Mean FSH level was 17 IU/mL. In 10 of the cases AZFc deletion, in 1 case AZFb deletion and in 1 case both deletions were identified. Number of patients underwent microTESE and conventional TESE were 109 and 56, respectively. Regarding the pathological outcomes, 91 (55.2%) cases were germinal cell aplasia, 32 (19.4%) cases were maturation arrest, 23 (13.9%) cases were normal spermatogenesis, 10 (6.1%) cases were tubulary sclerosis, 9 (5.5%) cases were hipospermatogenesis. The cases in which Y microdeletion was present, pathological outcome was 75% germinal cell aplasia and 25% maturation arrest. Sperm avaliability was present in 37.5% of all cases, and this ratio was 27.2% in the cases with AZFc mutation.

Conclusion: TESE operation is a method that can be applied successfully in the treatment of azospermia. Detection of the AZF gene mutations is useful to predict a negative TESE outcome.

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